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CAPZA1 Mouse Monoclonal Antibody [A2E7]

Usd: 350

Specification

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Catalog# EM1901-82

CAPZA1 Mouse Monoclonal Antibody [A2E7]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CAPZA1 Mouse Monoclonal Antibody [A2E7]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human CAPZA1 aa 60-248 / 286.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 33 kDa

Positive Control

K-562 cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, HeLa, human colon cancer tissue.

Conjugation

unconjugated

Clone Number

A2E7

RRID

AB_3068927

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:1,000

Target

Function

CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.

Background References

1. Huang D. et. al. Hypoxia induces actin cytoskeleton remodeling by regulating the binding of CAPZA1 to F-actin via PIP2 to drive EMT in hepatocellular carcinoma. Cancer Lett. 2019 Apr 28;448:117-127.

2. Tsugawa H. et. al. Cancer Stem-Cell Marker CD44v9-Positive Cells Arise From Helicobacter pylori-Infected CAPZA1-Overexpressing Cells. Cell Mol Gastroenterol Hepatol. 2019;8(3):319-334.

Sequence Similarity

Belongs to the F-actin-capping protein alpha subunit family.

Subcellular Location

Cytoskeleton.

UNIPROT

SwissProt: P52907 Human

SwissProt: P47753 Mouse

SwissProt: B2GUZ5 Rat

Synonyms

Cap Z antibody

Cappa 1 antibody

Cappa1 antibody

Capping protein (actin filament) muscle Z line alpha 1 antibody

Capping protein alpha 1 antibody

Capping protein muscle Z line alpha 1 antibody

CapZ alpha 1 antibody

CapZ alpha-1 antibody

CAPZ antibody

CAPZA 1 antibody

Expand

Images

  • Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody (<a href="/products/EM1901-82" style="font-weight: bold;text-decoration: underline;">EM1901-82</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: HeLa cell lysate<br />Lane 5: HepG2 cell lysate<br />Lane 6: A549 cell lysate<br />Lane 7: NIH/3T3 cell lysate<br />Lane 8: RAW264.7 cell lysate<br />Lane 9: C2C12 cell lysate<br />Lane 10: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 46 seconds;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1901-82" style="font-weight: bold;text-decoration: underline;">EM1901-82</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/5,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: HeLa cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: A549 cell lysate
    Lane 7: NIH/3T3 cell lysate
    Lane 8: RAW264.7 cell lysate
    Lane 9: C2C12 cell lysate
    Lane 10: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

    Exposure time: 46 seconds;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-82) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling CAPZA1 with Mouse anti-CAPZA1 antibody (<a href="/products/EM1901-82" style="font-weight: bold;text-decoration: underline;">EM1901-82</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CAPZA1 antibody (<a href="/products/EM1901-82" style="font-weight: bold;text-decoration: underline;">EM1901-82</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling CAPZA1 with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CAPZA1 antibody (<a href="/products/EM1901-82" style="font-weight: bold;text-decoration: underline;">EM1901-82</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-82" style="font-weight: bold;text-decoration: underline;">EM1901-82</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CAPZA1 antibody (EM1901-82) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-82) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"