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Lysozyme Mouse Monoclonal Antibody [A5A8]

Usd: 350

Specification

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Catalog# EM1901-98

Lysozyme Mouse Monoclonal Antibody [A5A8]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Lysozyme Mouse Monoclonal Antibody [A5A8]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human Lysozyme aa 1-141 / 148.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 17 kDa

Positive Control

HepG2 cell lysate, HL-60 cell lysate, human spleen tissue, human stomach cancer tissue.

Conjugation

unconjugated

Clone Number

A5A8

RRID

AB_3068943

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

Target

Function

Lysozyme, also known as muramidase or N-acetylmuramide glycanhydrolase, is an antimicrobial enzyme produced by animals that forms part of the innate immune system. Lysozyme is a glycoside hydrolase that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is the major component of gram-positive bacterial cell wall. Lysozyme is abundant in secretions including tears, saliva, human milk, and mucus. It is also present in cytoplasmic granules of the macrophages and the polymorphonuclear neutrophils (PMNs). Large amounts of lysozyme can be found in egg white. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure, making them part of the same glycoside hydrolase family 22. In humans, the C-type lysozyme enzyme is encoded by the LYZ gene. In certain cancers (especially myelomonocytic leukemia) excessive production of lysozyme by cancer cells can lead to toxic levels of lysozyme in the blood. High lysozyme blood levels can lead to kidney failure and low blood potassium, conditions that may improve or resolve with treatment of the primary malignancy. Serum lysozyme is much less specific for diagnosis of sarcoidosis than serum angiotensin converting enzyme; however, since it is more sensitive, it is used as a marker of sarcoidosis disease activity and is suitable for disease monitoring in proven cases.

Background References

1. Wu T. et. al. What is new in lysozyme research and its application in food industry? A review. Food Chem. 2019 Feb.

2. Liu J. et. al. The antimicrobial spectrum of lysozyme broadened by reductive modification. Poult Sci. 2018 Nov.

Sequence Similarity

Belongs to the glycosyl hydrolase 22 family.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P61626 Human

Synonyms

1 4 beta N acetylmuramidase C antibody

1 antibody

4-beta-N-acetylmuramidase C antibody

EC 3.2.1.17 antibody

LYSC_HUMAN antibody

Lysosyme antibody

Lysozyme (renal amyloidosis) antibody

Lysozyme C antibody

Lysozyme C precursor antibody

LYZ antibody

Expand

Images

  • Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (<a href="/products/EM1901-98" style="font-weight: bold;text-decoration: underline;">EM1901-98</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HL-60 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1901-98" style="font-weight: bold;text-decoration: underline;">EM1901-98</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Lysozyme on different lysates with Mouse anti-Lysozyme antibody (EM1901-98) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HL-60 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 14 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-98) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (<a href="/products/EM1901-98" style="font-weight: bold;text-decoration: underline;">EM1901-98</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-98" style="font-weight: bold;text-decoration: underline;">EM1901-98</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-Lysozyme antibody (EM1901-98) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Lysozyme antibody (<a href="/products/EM1901-98" style="font-weight: bold;text-decoration: underline;">EM1901-98</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-98" style="font-weight: bold;text-decoration: underline;">EM1901-98</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-Lysozyme antibody (EM1901-98) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"