ddx24 Mouse Monoclonal Antibody [A3B10]
Catalog# EM1902-03
ddx24 Mouse Monoclonal Antibody [A3B10]
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IHC-P
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IF-Tissue
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Zebrafish
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unconjugated
Overview
Product Name
ddx24 Mouse Monoclonal Antibody [A3B10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within zebrafish ddx24 aa 562-819 / 832.
Species Reactivity
Zebrafish
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
94 kDa
Positive Control
Zebrafish tissue.
Conjugation
unconjugated
Clone Number
A3B10
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein G affinity purified.
Application Dilution
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IHC-P
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1:100-1:500
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IF-Tissue
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1:200
Target
Function
ATP-dependent RNA helicase DDX24 is an enzyme that in humans is encoded by the DDX24 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial RNA splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which shows little similarity to any of the other known human DEAD box proteins, but shows a high similarity to mouse Ddx24 at the amino acid level.
Background References
1. Pang P. et. al. DDX24 Mutations Associated With Malformations of Major Vessels to the Viscera. Hepatology. 2019 Feb;69(2):803-816.
2. Shi D. et. al. Negative regulation of the p300-p53 interplay by DDX24. Oncogene. 2016 Jan 28;35(4):528-36.
Subcellular Location
Nucleolus.
UNIPROT
Synonyms
DEAD (Asp-Glu-Ala-Asp) box helicase 24
ddx24
Images
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Immunofluorescence analysis of paraffin-embedded zebrafish tissue labeling ddx24 with Mouse anti-ddx24 antibody (EM1902-03) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1902-03, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-ddx24 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-03, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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