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Myc tag Mouse Monoclonal Antibody [A3C8]

Usd: 350

Specification

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Catalog# EM1902-38

Myc tag Mouse Monoclonal Antibody [A3C8]

Application

  • WB

  • ELISA

  • IP

  • IF-Cell

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Myc tag Mouse Monoclonal Antibody [A3C8]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin.

Species Reactivity

Species independent

Validated Applications

WB, ELISA, IP, IF-Cell

Positive Control

C-terminal Myc-tagged recombinant protein lysates, N-terminal Myc-tagged recombinant protein lysates.

Conjugation

unconjugated

Clone Number

A3C8

RRID

AB_3068982

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:50,000-1:100,000

  • IF-Cell

  • 1:1,000

  • IP

  • 2-5 µg/ml.

  • ELISA

  • Use at an assay dependent concentration.

Target

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis . Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).Myc proteins are transcription factors that activate expression of many pro-proliferative genes through binding enhancer box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc is thought to function by upregulating transcript elongation of actively transcribed genes through the recruitment of elongation factors.[8] It can also act as a transcriptional repressor. By binding Miz-1 transcription factor and displacing the p300 co-activator, it inhibits expression of Miz-1 target genes. In addition, myc has a direct role in the control of DNA replication.[9] This activity could contribute to DNA amplification in cancer cells.

Background References

1. Shi Y. et. al. tRNA synthetase counteracts c-Myc to develop functional vasculature. Elife 3:E02349-E02349(2014) .

Synonyms

AU016757 antibody

Avian myelocytomatosis viral oncogene homolog antibody

bHLHe39 antibody

c Myc antibody

Cellular myelocytomatosis oncogene antibody

Class E basic helix-loop-helix protein 39 antibody

MGC105490 antibody

MRTL antibody

Myc antibody

Myc protein antibody

Expand

Images

  • Western blot analysis of Myc tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a>, 1/100,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:10,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myc tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/100,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:10,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Myc tag on N-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a>, 1/100,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:10,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Myc tag on N-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/100,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:10,000 dilution was used for 1 hour at room temperature.

  • Immobilized N-terminal Myc-tagged recombinant protein. at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.

    Immobilized N-terminal Myc-tagged recombinant protein. at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.

  • Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: Myc Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> IP in Myc Tag fusion protein lysate.<br />Lane 3: <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> IP in Myc Tag fusion protein lysate.<br />Lane 4: Mouse IgG instead of <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> in Myc Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 60 mins at room temperature.

    Lane 1: Myc Tag fusion protein lysate (input).
    Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
    Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
    Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using <a href="/products/R1208-1" style="font-weight: bold;text-decoration: underline;">R1208-1</a> at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 60 mins at room temperature.<br /><br />Lane 1: Myc Tag fusion protein lysate (input).<br />Lane 2: <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> IP in Myc Tag fusion protein lysate.<br />Lane 3: <a href="/products/EM31105" style="font-weight: bold;text-decoration: underline;">EM31105</a> IP in Myc Tag fusion protein lysate.<br />Lane 4: Mouse IgG instead of <a href="/products/EM1902-38" style="font-weight: bold;text-decoration: underline;">EM1902-38</a> in Myc Tag fusion protein lysate.<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST

    Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 60 mins at room temperature.

    Lane 1: Myc Tag fusion protein lysate (input).
    Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
    Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
    Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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