HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]
Catalog# EM21103
HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human HSP90 Beta aa 311-360 / 724.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, human brain tissue, rat brain tissue, human colon carcinoma tissue, human tonsil tissue, mouse testis tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
2-1-G3
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:500-1:10,000
Target
Function
Heat shock protein HSP 90-beta also called HSP90beta is a protein that in humans is encoded by the HSP90AB1 gene. HSP90AB1 is a molecular chaperone. Chaperones are proteins that bind to other proteins, thereby stabilizing them in an ATP-dependent manner. Chaperones stabilize new proteins during translation, mature proteins which are partially unstable but also proteins that have become partially denatured due to various kinds of cellular stress. In case proper folding or refolding is impossible, HSPs mediate protein degradation. They also have specialized functions, such as intracellular transport into organelles.
Background References
1. "Hsp90 is regulated by a switch point in the C-terminal domain."Retzlaff M., Stahl M., Eberl H.C., Lagleder S., Beck J., Kessler H., Buchner J.EMBO Rep. 10:1147-1153(2009).
2. "GCUNC-45 is a novel regulator for the progesterone receptor/hsp90 chaperoning pathway."Chadli A., Graham J.D., Abel M.G., Jackson T.A., Gordon D.F., Wood W.M., Mol. Cell. Biol. 26:1722-1730(2006)
Sequence Similarity
Belongs to the heat shock protein 90 family.
Post-translational Modification
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).; ISGylated.; S-nitrosylated; negatively regulates the ATPase activity.; Phosphorylation at Tyr-301 by SRC is induced by lipopolysaccharide. Phosphorylation at Ser-226 and Ser-255 inhibits AHR interaction.; Methylated by SMYD2; facilitates dimerization and chaperone complex formation; promotes cancer cell proliferation.; Cleaved following oxidative stress resulting in HSP90AB1 protein radicals formation; disrupts the chaperoning function and the degradation of its client proteins.
Subcellular Location
Cytoplasm
Synonyms
90 kda heat shock protein beta HSP90 beta antibody
D6S182 antibody
FLJ26984 antibody
Heat shock 84 kDa antibody
Heat shock 90kD protein 1, beta antibody
Heat shock 90kDa protein 1 beta antibody
Heat shock protein 90 alpha family class B member 1 antibody
Heat shock protein 90 kDa antibody
Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
Heat shock protein 90kDa alpha family class B member 1 antibody
ExpandImages
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Western blot analysis of HSP90 Beta on different lysates with Mouse anti-HSP90 Beta antibody (EM21103) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Mouse heart tissue lysate (40 µg/Lane)
Lane 11: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 53 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21103) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 Beta antibody (EM21103) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-HSP90 Beta antibody (EM21103) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
Citation
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Inhibition of herpes simplex virus infection by Caesappanin C targeting virus gB protein and host HSP90β
Journal: Phytomedicine
DOI: 10.1016/j.phymed.2026.157802
IF: 8.3
Application: CoIP
Reactivity: Mouse
Publish date: 2026 Jan
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Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus
Journal: Frontiers In Microbiology
DOI:
IF: 4.235
Application: WB,IP,Co-IP
Reactivity: Chicken
Publish date: 2020 Jul
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
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Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
HSP90 Beta Recombinant Mouse Monoclonal Antibody [2-1-G3-R]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
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Conjugate: unconjugated
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Application: WB,IHC-P,IF-Cell
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Conjugate: unconjugated
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Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated