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T7 tag Mouse Monoclonal Antibody [4-C2]

Usd: 350

Specification

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Catalog# EM50802

T7 tag Mouse Monoclonal Antibody [4-C2]

Application

  • WB

  • ELISA

  • IP

  • IF-Cell

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

T7 tag Mouse Monoclonal Antibody [4-C2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide CMASMTGGQQMG.

Species Reactivity

Species independent

Validated Applications

WB, ELISA, IP, IF-Cell

Positive Control

T7-tagged recombinant protein.

Conjugation

unconjugated

Clone Number

4-C2

RRID

AB_3069005

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • ELISA

  • Use at an assay dependent concentration.

  • IF-Cell

  • Use at an assay dependent concentration.

  • IP

  • Use at an assay dependent concentration.

Target

Function

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. This antibody recognizes the T7-tag fused to either the amino- or carboxy-terminus of targeted proteins.

Background References

1. Horn AV et al. Human LINE-1 restriction by APOBEC3C is deaminase independent and mediated by an ORF1p interaction that affects LINE reverse transcriptase activity. Nucleic Acids Res N/A:N/A (2013)

2. Kubota T., et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem. 283:25660-70 (2008).

Synonyms

10 antibody

Major capsid protein 10A antibody

Major capsid protein antibody

Minor capsid protein 10B antibody

T7 epitope tag antibody

Images

  • Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (<a href="/products/EM50802" style="font-weight: bold;text-decoration: underline;">EM50802</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 43 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM50802" style="font-weight: bold;text-decoration: underline;">EM50802</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (EM50802) at 1/1,000 dilution.

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 43 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50802) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation