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GST Mouse Monoclonal Antibody [16-21]

Usd: 350

Specification

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Catalog# EM80701

GST Mouse Monoclonal Antibody [16-21]

Application

  • WB

  • ELISA

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GST Mouse Monoclonal Antibody [16-21]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within GST protein.

Species Reactivity

Species independent

Validated Applications

WB, ELISA

Positive Control

GST-tagged recombinant protein.

Conjugation

unconjugated

Clone Number

16-21

RRID

AB_2940896

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • ELISA

  • 1:10,000-1:20,000

Target

Function

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.

Background References

1. Choi, H.K., et al. 2013. Early estrogen-induced gene 1, a novel RANK signaling component, is essential for osteoclastogenesis. Cell Res. 23: 524-536.

2. Patra, A.K., et al. 2013. An alternative NFAT-activation pathway mediated by IL-7 is critical for early thymocyte development. Nat. Immunol. 14: 127-135.

Synonyms

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

Images

  • Western blot analysis of GST on different lysates with Mouse anti-GST antibody (<a href="/products/EM80701" style="font-weight: bold;text-decoration: underline;">EM80701</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 46 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM80701" style="font-weight: bold;text-decoration: underline;">EM80701</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GST on different lysates with Mouse anti-GST antibody (EM80701) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 46 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM80701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation