LGR5 Rabbit Polyclonal Antibody
Catalog# ER10708
LGR5 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LGR5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Mouse GPR49 / LGR5 alpha / beta aa 1-50 / 907 (ID: Q9Z1P4).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 100 kDa
Positive Control
Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, F9 cell lysate, Hela cell lysate, K562 cell lysate.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Background References
1. Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors."McClanahan T., Koseoglu S., Smith K., Grein J., Gustafson E., Black S., Kirschmeier P., Samatar A.A.Cancer Biol. Ther. 5:419-426(2006)
2. "Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue."Becker L., Huang Q., Mashimo H.ScientificWorldJournal 8:1168-1176(2008))
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
Subcellular Location
Cytoplasm, cell membrane, Golgi apparatus.
Synonyms
FEX antibody
G protein coupled receptor 49 antibody
G protein coupled receptor 67 antibody
g protein-coupled receptor fex antibody
G-protein coupled receptor 49 antibody
G-protein coupled receptor 67 antibody
G-protein coupled receptor HG38 antibody
GPR 49 antibody
GPR 67 antibody
GPR49 antibody
ExpandImages
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Western blot analysis of LGR5 on different lysates with Rabbit anti-LGR5 antibody (ER10708) at 1/1,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER10708) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of LGR5 on different cell lysates using anti- GPR49/LGR5 alpha/beta antibody at 1/1,000 dilution.
Positive control:
Lane 1: F9 cell lysate
Lane 2: Hela cell lysate
Lane 3: K562 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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