NaV1.8 Rabbit Polyclonal Antibody
Catalog# ER1706-30
NaV1.8 Rabbit Polyclonal Antibody
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IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
NaV1.8 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within rat NaV18 aa 1,907-1,956 / 1,956.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 220 kDa
Positive Control
Mouse brain tissue, Rat Dorsal root ganglia.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:500
Target
Function
Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
Background References
1. Christidis N et al. Expression of 5-HT3 receptors and TTX resistant sodium channels (Na(V)1.8) on muscle nerve fibers in pain-free humans and patients with chronic myofascial temporomandibular disorders. J Headache Pain 15:63 (2014).
2. Cejudo-Roman A et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013).
Sequence Similarity
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
Tissue Specificity
Expressed in dorsal root ganglion and trigeminal ganglion.
Post-translational Modification
Ubiquitinated by NEDD4L; which promotes its endocytosis.; Phosphorylation at Ser-1452 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.; Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 815) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4.
Subcellular Location
Plasma membrane.
Synonyms
hPN3 antibody
mPN3 antibody
Peripheral nerve sodium channel 3 antibody
Pn3 (gene name) antibody
PN3 antibody
SCN10A antibody
SCNAA_HUMAN antibody
Sensory neuron sodium channel antibody
Sns (gene name) antibody
SNS antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NaV1.8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Dorsal root ganglia
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ER1706-30, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"