SATB1 Rabbit Polyclonal Antibody
Catalog# ER1802-24
SATB1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
SATB1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within N-terminal human SATB1 aa.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size 86 kDa
Positive Control
Mouse thymus tissue lysate,SH-SY-5Y, Jurkat.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:100
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FC
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1:50-1:100
Target
Function
The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.
Background References
暂无
Sequence Similarity
Belongs to the CUT homeobox family.
Tissue Specificity
Expressed predominantly in thymus.
Post-translational Modification
Sumoylated. Sumoylation promotes cleavage by caspases.; Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.; Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms cannot dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
Subcellular Location
Nucleus.
Synonyms
DNA binding protein SATB1 antibody
DNA-binding protein SATB1 antibody
SATB homeobox 1 antibody
SATB1 antibody
SATB1_HUMAN antibody
Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
Special AT rich sequence binding protein 1 antibody
Special AT-rich sequence-binding protein 1 antibody
Images
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ICC staining SATB1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western blot analysis of SATB1 on different lysates with Rabbit anti-SATB1 antibody (ER1802-24) at 1/1,000 dilution.
Lane 1: THP-1(Human acute monoblastic leukemia cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-24, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 86 kDa
Observed band size: 86 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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