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BNIP1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-66

BNIP1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

BNIP1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human BN1P1 aa 27-228 / 228.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 26 kDa

Positive Control

Rat liver tissue lysate, HepG2, LOVO, human liver tissue, human placenta tissue.

Conjugation

unconjugated

RRID

AB_3069164

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:100

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:500

Target

Function

The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria.

Background References

1. Nakajima K et al. Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. EMBO J 23:3216-3226 (2004).

2. Tang F et al. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. PLoS ONE 6:E24367-E24367 (2011).

Sequence Similarity

Belongs to the SEC20 family.

Tissue Specificity

Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.

Post-translational Modification

Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.

Subcellular Location

Endoplasmic reticulum membrane, Mitochondrion membrane.

UNIPROT

SwissProt: Q12981 Human

SwissProt: Q8VHI8 Rat

Synonyms

BCL2 adenovirus E1B 19kD interacting protein 1 antibody

BCL2/adenovirus E1B 19 kDa protein-interacting protein 1 antibody

BNIP1 antibody

MGC41600 antibody

Nip1 antibody

OTTHUMP00000161077 antibody

OTTHUMP00000161078 antibody

OTTHUMP00000161079 antibody

OTTHUMP00000223580 antibody

sec20 antibody

Expand

Images

  • Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.

    Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.

  • ICC staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BNIP1 antibody (<a href="/products/ER1802-66" style="font-weight: bold;text-decoration: underline;">ER1802-66</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1802-66" style="font-weight: bold;text-decoration: underline;">ER1802-66</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BNIP1 antibody (ER1802-66) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-66) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"