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KV4.3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1802-91

KV4.3 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

KV4.3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human Kv43 aa 435-484 / 655.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 73 kDa

Positive Control

MCF-7 cell lysate, mouse marrow tissue lysate, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue.

Conjugation

unconjugated

RRID

AB_3069191

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes – shaker, shaw, shab, and shal – have been identified in Drosophila, and each has been shown to have human homolog(s). Kv4.3 is a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene.

Background References

暂无

Sequence Similarity

Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.

Tissue Specificity

Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.

Post-translational Modification

Regulated through phosphorylation at Ser-569 by CaMK2D.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q9UK17 Human

SwissProt: Q9Z0V1 Mouse

Synonyms

KCND 3 antibody

KCND 3L antibody

KCND 3S antibody

Kcnd3 antibody

KCND3_HUMAN antibody

KCND3L antibody

KCND3S antibody

KSHIVB antibody

Kv 4.3 antibody

MGC14203 antibody

Expand

Images

  • Western blot analysis of KV4.3 on different lysates with Rabbit anti-KV4.3 antibody (<a href="/products/ER1802-91" style="font-weight: bold;text-decoration: underline;">ER1802-91</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1802-91" style="font-weight: bold;text-decoration: underline;">ER1802-91</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 73 kDa<br />Observed band size: 72 kDa

    Western blot analysis of KV4.3 on different lysates with Rabbit anti-KV4.3 antibody (ER1802-91) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1802-91, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 73 kDa
    Observed band size: 72 kDa

  • ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"