USP13 Rabbit Polyclonal Antibody
Catalog# ER1803-09
USP13 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
USP13 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human USP13 aa 570-776 / 863.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC, IHC-P
Molecular Weight
97 kDa
Positive Control
293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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FC
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1:50-1:100
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IHC-P
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1:100-1:200
Target
Function
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.
Background References
1. Liu J et al. Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 147:223-234 (2011).
2. Scortegagna M et al. USP13 enzyme regulates Siah2 ligase stability and activity via noncatalytic ubiquitin-binding domains. J Biol Chem 286:27333-27341 (2011).
Sequence Similarity
Belongs to the peptidase C19 family.
Tissue Specificity
Highly expressed in ovary and testes.
Subcellular Location
Nucleus. Cytosol.
Synonyms
Deubiquitinating enzyme 13 antibody
Isopeptidase T 3 antibody
Isopeptidase T-3 antibody
Isopeptidase T3 antibody
ISOT 3 antibody
ISOT-3 antibody
ISOT3 antibody
Ubiquitin carboxyl terminal hydrolase 13 antibody
Ubiquitin carboxyl-terminal hydrolase 13 antibody
Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of USP13 on different lysates with Rabbit anti-USP13 antibody (ER1803-09) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-USP13 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-09) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain -
ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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