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Orai3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-12

Orai3 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Orai3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Human Orai-3 aa 11-60 / 295.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

31 kDa

Positive Control

Raji, A549, MG-63, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human kidney tissue, human placenta tissue, mouse testis tissue.

Conjugation

unconjugated

RRID

AB_3069211

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3) is a Protein Coding gene. Among its related pathways are Calcium signaling pathway. Gene Ontology (GO) annotations related to this gene include store-operated calcium channel activity. An important paralog of this gene is ORAI2.

Background References

1. Srikanth S et al. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat Cell Biol 12:436-446 (2010).

Sequence Similarity

Belongs to the Orai family.

Tissue Specificity

Expressed in both naive and effector T helper cells with higher levels in effector cells.

Subcellular Location

Membrane. Multi-pass membrane protein.

UNIPROT

SwissProt: Q9BRQ5 Human

SwissProt: Q6P8G8 Mouse

SwissProt: Q6AXR8 Rat

Synonyms

MGC13024 antibody

Orai 3 antibody

ORAI calcium release activated calcium modulator 3 antibody

Orai3 antibody

ORAI3_HUMAN antibody

Protein orai 3 antibody

Protein orai-3 antibody

TMEM 142C antibody

TMEM142C antibody

Transmembrane protein 142C antibody

Images

  • ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.

  • Western blot analysis of Orai3 on different lysates with Rabbit anti-Orai3 antibody (<a href="/products/ER1803-12" style="font-weight: bold;text-decoration: underline;">ER1803-12</a>) at 1/500 dilution.<br /><br />Lane 1: MG-63 (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: SH-SY5Y (Human neuroblastoma cells) cell lysate<br />Lane 3: MCF7 (Human breast cancer cells) cell lysate<br />Lane 4: NIH/3T3 (Mouse fibroblast cells) cell lysate <br />Lane 5: Mouse testis tissue lysate<br />Lane 6: Rat testis tissue lysate<br /><br />Lysates/proteins at cell:20ug/lane,tissue:40ug/lane.<br />Exposure time: 15 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-12" style="font-weight: bold;text-decoration: underline;">ER1803-12</a>, 1/500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 31 kDa<br />Observed band size: 40 kDa

    Western blot analysis of Orai3 on different lysates with Rabbit anti-Orai3 antibody (ER1803-12) at 1/500 dilution.

    Lane 1: MG-63 (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: SH-SY5Y (Human neuroblastoma cells) cell lysate
    Lane 3: MCF7 (Human breast cancer cells) cell lysate
    Lane 4: NIH/3T3 (Mouse fibroblast cells) cell lysate
    Lane 5: Mouse testis tissue lysate
    Lane 6: Rat testis tissue lysate

    Lysates/proteins at cell:20ug/lane,tissue:40ug/lane.
    Exposure time: 15 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-12, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 31 kDa
    Observed band size: 40 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"