Eg5 Rabbit Polyclonal Antibody
Catalog# ER1803-25
Eg5 Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Eg5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human Eg5 aa 80-257 / 1,056.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 119 kDa
Positive Control
A431, SH-SY-5Y, SiHa, rat kidney tissue, human lung cancer tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50
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IHC-P
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1:50-1:400
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FC
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1:50-1:100
Target
Function
Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.
Background References
暂无
Sequence Similarity
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
Post-translational Modification
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).; A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Subcellular Location
Cytoplasm, Cytoskeleton.
Synonyms
EG5 antibody
HKSP antibody
KIF11 antibody
KIF11_HUMAN antibody
Kinesin family member 11 antibody
Kinesin like protein 1 antibody
Kinesin-like protein 1 antibody
Kinesin-like protein KIF11 antibody
Kinesin-like spindle protein HKSP antibody
Kinesin-related motor protein Eg5 antibody
ExpandImages
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ICC staining Eg5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Eg5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Eg5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Eg5 antibody (ER1803-25) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-25) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Eg5 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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