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CACNG1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-27

CACNG1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CACNG1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse CACNG1 aa 13-62 / 223.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, FC

Target Molecular Weight

Predicted band size: 25 kDa

Positive Control

Human skeletal muscle lysates, human fetal skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, SH-SY5Y.

Conjugation

unconjugated

RRID

AB_3069225

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Regulates channel inactivation kinetics.

Background References

1. Powers P A et al.Molecular characterization of the gene encoding the gamma subunit of the human skeletal muscle 1,4-dihydropyridine-sensitive Ca2+ channel (CACNLG), cDNA sequence, gene structure, and chromosomal location. J Biol Chem 268:9275-9279 (1993).

2. Iles D E et al. Localization of the gamma-subunit of the skeletal muscle L-type voltage-dependent calcium channel gene (CACNLG) to human chromosome band 17q24 by in situ hybridization and identification of a polymorphic repetitive DNA sequence at the gene locus. Cytogenet Cell Genet 64:227-230 (1993).

Sequence Similarity

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

Tissue Specificity

Skeletal muscle.

Post-translational Modification

N-glycosylated.

Subcellular Location

Cell membrane, sarcolemma.

UNIPROT

SwissProt: Q06432 Human

SwissProt: O70578 Mouse

SwissProt: P97707 Rat

Synonyms

CACNG1 antibody

CACNLG antibody

CACNLG calcium channel neuronal dihydrpyridine sensitive gamma subunit antibody

Calcium channel L type gamma polypeptide antibody

Calcium channel voltage dependent gamma subunit 1 antibody

CCG1_HUMAN antibody

Dihydropyridine sensitive L type skeletal muscle calcium channel gamma subunit antibody

Dihydropyridine sensitive L type skeletal muscle calcium channel subunit gamma antibody

Dihydropyridine-sensitive L-type antibody

fb43f02 antibody

Expand

Images

  • Western blot analysis of CACNG1 on human skeletal muscle lysate using anti-CACNG1 antibody at 1/1,000 dilution.

    Western blot analysis of CACNG1 on human skeletal muscle lysate using anti-CACNG1 antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling CACNG1 with Rabbit anti-CACNG1 antibody (<a href="/products/ER1803-27" style="font-weight: bold;text-decoration: underline;">ER1803-27</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CACNG1 antibody (<a href="/products/ER1803-27" style="font-weight: bold;text-decoration: underline;">ER1803-27</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SH-SY5Y cells labeling CACNG1 with Rabbit anti-CACNG1 antibody (ER1803-27) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CACNG1 antibody (ER1803-27) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of SH-SY5Y cells labeling CACNG1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ER1803-27" style="font-weight: bold;text-decoration: underline;">ER1803-27</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SH-SY5Y cells labeling CACNG1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-27, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"