IL12B Rabbit Polyclonal Antibody
Catalog# ER1803-31
IL12B Rabbit Polyclonal Antibody
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WB
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Mouse
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unconjugated
Overview
Product Name
IL12B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse IL12B aa 220-300.
Species Reactivity
Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 37 kDa
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:5,000-1:10,000
Target
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Background References
暂无
Sequence Similarity
Belongs to the IL-12B family.
Post-translational Modification
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Subcellular Location
Secreted.
UNIPROT
Synonyms
CLMF antibody
CLMF p40 antibody
CLMF2 antibody
Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
IL 12B antibody
IL-12 subunit p40 antibody
IL-12B antibody
IL12 p40 antibody
IL12B antibody
IL12B_HUMAN antibody
ExpandImages
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Western blot analysis of IL12B on different lysates with Rabbit anti-IL12B antibody (ER1803-31) at 1/5,000 dilution.
Lane 1: His-tagged Mouse IL12B recombinant protein (50 ng/Lane)
Lane 2: His-tagged Human IL12B recombinant protein (200 ng/Lane)
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-31) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"