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CACNG3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1803-38

CACNG3 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CACNG3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within rat CACNG3 aa 192-241 / 315.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size 36 kDa

Positive Control

SH-SY-5Y, rat brain tissue.

Conjugation

unconjugated

RRID

AB_3069234

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Regulates the trafficking to the somatodendritic compartment and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.

Background References

1. Spencer KL et al. Dissection of chromosome 16p12 linkage peak suggests a possible role for CACNG3 variants in age-related macular degeneration susceptibility. Invest Ophthalmol Vis Sci. 28;52(3):1748-54 (2011).

2. Everett KV et al. Linkage and association analysis of CACNG3 in childhood absence epilepsy. Eur J Hum Genet. 15(4):463-72 (2007).

Sequence Similarity

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.

Subcellular Location

Membrane.

UNIPROT

SwissProt: O60359 Human

SwissProt: Q8VHX0 Rat

Synonyms

CACNG 3 antibody

Cacng2 antibody

CACNG3 antibody

Calcium channel voltage dependent gamma subunit 3 antibody

CCG3_HUMAN antibody

Neuronal voltage gated calcium channel gamma 3 subunit antibody

Neuronal voltage-gated calcium channel gamma-3 subunit antibody

TARP gamma-3 antibody

Transmembrane AMPAR regulatory protein gamma-3 antibody

Voltage dependent calcium channel gamma 3 subunit antibody

Expand

Images

  • Western blot analysis of CACNG3 on different lysates with Rabbit anti-CACNG3 antibody (<a href="/products/ER1803-38" style="font-weight: bold;text-decoration: underline;">ER1803-38</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1803-38" style="font-weight: bold;text-decoration: underline;">ER1803-38</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 36 kDa<br />Observed band size: 50 kDa

    Western blot analysis of CACNG3 on different lysates with Rabbit anti-CACNG3 antibody (ER1803-38) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1803-38, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 36 kDa
    Observed band size: 50 kDa

  • ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"