Hemagglutinin-neuraminidase Rabbit Polyclonal Antibody
Catalog# ER1803-50
Hemagglutinin-neuraminidase Rabbit Polyclonal Antibody
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WB
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ELISA
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Avian avulavirus 1
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unconjugated
Overview
Product Name
Hemagglutinin-neuraminidase Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within newcastle disease virus Hemagglutinin-neuraminidase aa 206-255 / 586.
Species Reactivity
Avian avulavirus 1
Validated Applications
WB, ELISA
Target Molecular Weight
Predicted band size: 64 kDa
Positive Control
Hemagglutinin-neuraminidase transfected HEK293 cell.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1,000
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ELISA
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1:5,000-1:10,000
Target
Function
Hemagglutinin-neuraminidase refers to a single viral protein that has both hemagglutinin and neuraminidase activity. This is in contrast to the proteins found in influenza, where both functions exist but in two separate proteins. However it does show a structural similarity to influenza viral neuraminidase and has a six-bladed beta-propeller structure. Hemagglutinin-neuraminidase allows the virus to stick to a potential host cell, and cut itself loose if necessary. Hemagglutinin-neuraminidase can be found in a variety of paramyxoviruses including mumps virus, human parainfluenza virus 3, and the avian pathogen Newcastle disease virus.
Background References
1. Zaitsev V et al. Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion. J Virol. 2004 Apr;78(7):3733-41.
2. Lawrence MC et al. Structure of the haemagglutinin-neuraminidase from human parainfluenza virus type III. J Mol Biol. 2004 Jan 30;335(5):1343-57.
Sequence Similarity
Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.
Subcellular Location
Virion membrane.
Images
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Western blot analysis of Hemagglutinin-neuraminidase on HEK293 cell lysates with Rabbit anti-Hemagglutinin-neuraminidase antibody (ER1803-50) at 1/5,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1803-50, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 64 kDa
Observed band size: 64 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"