USP14 Rabbit Polyclonal Antibody
Catalog# ER1803-55
USP14 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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unconjugated
Overview
Product Name
USP14 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human USP14 aa 226-408 / 494.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
Daudi cell lysates, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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FC
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1:50-1:100
Target
Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions. Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
Background References
1. Koulich E et al. Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome. Mol Biol Cell 19:1072-1082(2008).
2. Nagai A et al. USP14 inhibits ER-associated degradation via interaction with IRE1alpha. Biochem Biophys Res Commun 379:995-1000(2009).
Sequence Similarity
Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
Subcellular Location
Plasma membrane.
UNIPROT
Synonyms
Deubiquitinating enzyme 14 antibody
TGT antibody
tRNA guanine transglycosylase 60 kD subunit antibody
tRNA guanine transglycosylase antibody
Ubiquitin carboxyl terminal hydrolase 14 antibody
Ubiquitin carboxyl-terminal hydrolase 14 antibody
Ubiquitin specific peptidase 14 antibody
Ubiquitin specific processing protease 14 antibody
Ubiquitin specific protease 14 antibody
Ubiquitin thiolesterase 14 antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ER1803-55) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si USP14 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of USP14 on Daudi cell lysate using anti-USP14 antibody at 1/500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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