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Rice Os01g0847700 protein Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1901-09

Rice Os01g0847700 protein Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Rice

Conjugation

  • unconjugated

Overview

Product Name

Rice Os01g0847700 protein Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Rice Os01g0847700 aa 262-311 / 311.

Species Reactivity

Rice

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Rice stem.

Conjugation

unconjugated

RRID

AB_3069304

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Has oxidoreductase activity.

Background References

1. Narawongsanont R et al. Cloning and characterization of AKR4C14, a rice aldo-keto reductase, from Thai Jasmine rice. Protein J. 31(1):35-42 (2012).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q0JHR5 Rice

Images

  • Western blot analysis of Rice Os01g0847700 protein on rice stem lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER1901-09" style="font-weight: bold;text-decoration: underline;">ER1901-09</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Rice Os01g0847700 protein on rice stem lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • IF staining of Rice Os01g0847700 protein in rice stem tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    IF staining of Rice Os01g0847700 protein in rice stem tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rice stem tissue using anti-Rice Os01g0847700 protein antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rice stem tissue using anti-Rice Os01g0847700 protein antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"