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KCNAB1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1901-34

KCNAB1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

KCNAB1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse KCNAB1 aa 36-85 / 401.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 46.5 kDa

Positive Control

Mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, EA.hy926, SH-SY5Y.

Conjugation

unconjugated

RRID

AB_3069326

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:100

  • FC

  • 1:100-1:500

Target

Function

Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits. Modulates action potentials via its effect on the pore-forming alpha subunits (Probable). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members. Promotes the closure of KCNA1, KCNA2 and KCNA5 channels. Accelerates KCNA4 channel closure. Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4. Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6. Enhances KCNB1 and KCNB2 channel activity (By similarity). Binds NADPH; this is required for efficient down-regulation of potassium channel activity. Has NADPH-dependent aldoketoreductase activity. Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity.

Background References

1. Pan Y. et. al. Oxidation of NADPH on Kvbeta1 inhibits ball-and-chain type inactivation by restraining the chain. Proc. Natl. Acad. Sci. U.S.A. 108:5885-5890(2011).

2. Schulte U. et. al. The epilepsy-linked Lgi1 protein assembles into presynaptic Kv1 channels and inhibits inactivation by Kvbeta1. Neuron 49:697-706(2006).

Sequence Similarity

Belongs to the shaker potassium channel beta subunit family.

Tissue Specificity

In brain, expression is most prominent in caudate nucleus, hippocampus and thalamus. Significant expression also detected in amygdala and subthalamic nucleus. Also expressed in both healthy and cardiomyopathic heart. Up to four times more abundant in left ventricle than left atrium.

Subcellular Location

Cell membrane, cytoplasm, membrane.

UNIPROT

SwissProt: Q14722 Human

SwissProt: P63143 Mouse

SwissProt: P63144 Rat

Synonyms

hKvb3 antibody

hKvBeta3 antibody

K(+) channel subunit beta-1 antibody

KCAB1_HUMAN antibody

KCNA1B antibody

KCNAB1 antibody

Kv-beta-1 antibody

Kvb1.3 antibody

Voltage-gated potassium channel beta-1 subunit antibody

Voltage-gated potassium channel subunit beta-1 antibody

Images

  • Western blot analysis of KCNAB1 on different lysates with Rabbit anti-KCNAB1 antibody (<a href="/products/ER1901-34" style="font-weight: bold;text-decoration: underline;">ER1901-34</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 6 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER1901-34" style="font-weight: bold;text-decoration: underline;">ER1901-34</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 44.7 kDa<br />Observed band size: 60 kDa

    Western blot analysis of KCNAB1 on different lysates with Rabbit anti-KCNAB1 antibody (ER1901-34) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER1901-34, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 44.7 kDa
    Observed band size: 60 kDa

  • ICC staining of KCNAB1 in EA.hy926 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ER1901-34" style="font-weight: bold;text-decoration: underline;">ER1901-34</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of KCNAB1 in EA.hy926 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of KCNAB1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER1901-34" style="font-weight: bold;text-decoration: underline;">ER1901-34</a>, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of KCNAB1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-34, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"