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Arogenate dehydratase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1901-51

Arogenate dehydratase Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Rice

Conjugation

  • unconjugated

Overview

Product Name

Arogenate dehydratase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within rice Arogenate dehydratase aa 28-77 / 364.

Species Reactivity

Rice

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 40 kDa

Positive Control

Rice tissue lysates, rice tissue.

Conjugation

unconjugated

RRID

AB_3069341

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

Target

Function

Arogenate dehydratase (ADT) (EC 4.2.1.91) is an enzyme that catalyzes the chemical reaction. Hence, this enzyme has one substrate, L-arogenate, but 3 products:L-phenylalanine, H2O, and CO2. Certain forms of the protein have the potential to catalyze a second reaction. This enzyme participates in phenylalanine, tyrosine, and tryptophan biosynthesis. The carboxyl and hydroxide groups attached to the 2,5-cyclohexene ring are eliminated from L-arogenate, leaving as carbon dioxide and water. The 2,5-cyclohexene ring becomes a phenyl ring, and L-phenylalanine is formed. Certain forms of ADT have been shown to exhibit some prephenate dehydratase (PDT) activity in addition to the standard ADT activity described above. Known as cyclohexadienyl dehydratases or carbocyclohexadienyl dehydratases, these forms of the enzyme catalyze the same type of reaction (a decarboxylation and a dehydration) on prephenate. The carboxyl and hydroxide groups attached to the 2,5-cyclohexene ring are removed, leaving phenylpyruvate. ADT catalyzes a reaction categorized by two major changes in the structure of the substrate, these being a decarboxylation and a dehydration; the enzyme removes a carboxyl group and a water molecule (respectively). Both potential products of this reaction (L-arogenate and phenylpyruvate) occur at or near the end of the biosynthetic pathway. Total synthesis of L-arogenate has been reported.

Background References

1. Cho, Man-Ho; Corea, Oliver R. A.; Yang, Hong; Bedgar, Diana L.; Laskar, Dhrubojyoti D.; Anterola, Aldwin M.; et al. (2007). "Phenylalanine biosynthesis in Arabidopsis thaliana-- identification and characterization of arogenate dehydratases". J. Biol. Chem. 282 (42): 30827–35.

2. Maeda, H.; Shasany, A. K.; Schnepp, J.; Orlova, I.; Taguchi, G.; Cooper, B. R.; et al. (2010). "RNAi suppression of Arogenate Dehydratase 1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals". Plant Cell. 22 (3): 832–849.

Subcellular Location

Chloroplast stroma.

UNIPROT

SwissProt: Q6Z3Y3 Rice

SwissProt: ORYSJ Rice

Synonyms

Os07g0694600

Images

  • Western blot analysis of Arogenate dehydratase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER1901-51" style="font-weight: bold;text-decoration: underline;">ER1901-51</a>, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Arogenate dehydratase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-51, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Arogenate dehydratase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1901-51" style="font-weight: bold;text-decoration: underline;">ER1901-51</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Arogenate dehydratase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"