HDG11 Rabbit Polyclonal Antibody
Catalog# ER1901-56
HDG11 Rabbit Polyclonal Antibody
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WB
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IHC-P
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ELISA
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
HDG11 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within thaliana HDG11 aa 631-680 / 722.
Species Reactivity
Arabidopsis thaliana
Validated Applications
WB, IHC-P, ELISA
Target Molecular Weight
Predicted band size: 79 kDa
Positive Control
HDG11 recombinant protein, Arabidopsis thaliana tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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ELISA
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1:250-1:256,000
Target
Function
Transcription factor which acts as positive regulator of drought stress tolerance. Can transactivate CIPK3, NCED3 and ERECTA. Transactivates several cell-wall-loosening protein genes by directly binding to HD motifs in their promoters. These target genes play important roles in coordinating cell-wall extensibility with root development and growth. Transactivates CYP74A/AOS, AOC3, OPR3 and 4CLL5/OPCL1 genes by directly binding to HD motifs in their promoters. These target genes are involved in jasmonate (JA) biosynthesis, and JA signaling affects root architecture by activating auxin signaling, which promotes lateral root formation. Acts as negative regulator of trichome branching. Required for the establishment of giant cell identity on the abaxial side of sepals. May regulate cell differentiation and proliferation during root and shoot meristem development.
Background References
1. Guo XY. et. al. AtEDT1/HDG11 regulates stomatal density and water-use efficiency via ERECTA and E2Fa. New Phytol. 2019 Aug;223(3):1478-1488.
Sequence Similarity
Belongs to the HD-ZIP homeobox family. Class IV subfamily.
Tissue Specificity
Expressed in apical meristems and young epidermal tissue including trichomes and stipules. Expressed in lateral root tips, the L1 layer of apical inflorescence meristems and early flower primordia, carpel and petal epidermis, stigma papillae, ovule primordia, nucellus and embryo.
Subcellular Location
Nucleus.
Synonyms
Homeobox-leucine zipper protein HDG11
HD-ZIP protein HDG11
Homeodomain GLABRA 2-like protein 11
Homeodomain transcription factor HDG11
Protein ENHANCED DROUGHT TOLERANCE 1
Protein HOMEODOMAIN GLABROUS 11
HDG11
EDT1
HDGL2-11
At1g73360
ExpandImages
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Western blot analysis of HDG11 on HDG11 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-56) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HDG11 recombinant protein with primary antibody at 1:200 dilutions
Lane 2: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions -
Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-HDG11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"