Desmoglein 3 Rabbit Polyclonal Antibody
Catalog# ER1901-81
Desmoglein 3 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
-
IHC-P
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FC(Intra)
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Human
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Rat
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unconjugated
Overview
Product Name
Desmoglein 3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human Desmoglein 3 aa 301-615.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC(Intra)
Target Molecular Weight
Predicted band size: 108 kDa
Positive Control
HaCaT (Human immortalized keratinocyte) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
0.8 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:40,000
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IF-Cell
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1:200
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IHC-P
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1:800
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FC(Intra)
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1:1,000
Target
Function
Desmoglein-3 is a protein that in humans is encoded by the DSG3 gene. In the skin epidermis Desmoglein-3 is expressed in the basal lower layers of the epidermis, and dominates in terms of expression on mucosal surfaces compared to Desmoglein-1. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein, along with Desmoglein-1, has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. The mucosal dominant form of pemphigus vulgaris only involves antibodies against Desmoglein-3 and causes mucosal erosions, but no skin lesions. Desmoglein-3 serves as a prognostic marker of Esophageal Squamous Cell Carcinoma (ESCC), and may even be involved in the progression of ESCC.
Background References
1. Dworschak J et al. Mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides.J Dermatol Sci 65(2):102-9 (2012).
2. Savci-Heijink CD et al. The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung.Am J Pathol. 174(5):1629-37 (2009).
Tissue Specificity
Epidermis, tongue, tonsil, esophagus and carcinomas.
Subcellular Location
Cell membrane, Cell junction, desmosome, Cytoplasm, tight junction.
Synonyms
130 kD pemphigus vulgaris antigen antibody
130 kDa pemphigus vulgaris antigen antibody
Balding antibody
Cadherin family member 6 antibody
CDHF 6 antibody
CDHF6 antibody
Desmoglein 3 antibody
Desmoglein 3 precursor antibody
Desmoglein-3 antibody
Desmoglein3 antibody
ExpandImages
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Western blot analysis of Desmoglein 3 on different lysates with Rabbit anti-Desmoglein 3 antibody (ER1901-81) at 1/40,000 dilution.
Lane 1: HaCaT (Human immortalized keratinocyte) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1901-81, 1/40,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 108 kDa
Observed band size: 130 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: A431 (Human epidermoid carcinoma skin squamous cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: ER1901-81, 1/200, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Esophagus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ER1901-81, 1/800, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Esophagus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ER1901-81, 1/800, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Human
Sample: A431 (Human epidermoid carcinoma skin squamous cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: ER1901-81 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [PSH27-07]
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Desmoglein 3 Recombinant Rabbit Monoclonal Antibody [PSH27-06]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
