ENT1 Rabbit Polyclonal Antibody
Catalog# ER1902-23
ENT1 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
FC
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
ENT1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human ENT1 aa 19-68 / 456.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 50 kDa
Positive Control
Human plasma lysates, F9, JAR.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:50-1:100
-
FC
-
1:50-1:100
Target
Function
This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
Background References
1. Veras MA. et. al. Loss of ENT1 increases cell proliferation in the annulus fibrosus of the intervertebral disc. J Cell Physiol. 2019 Aug;234(8):13705-13719.
2. Kao YH. et. al. Targeting ENT1 and adenosine tone for the treatment of Huntington\'s disease. Hum Mol Genet. 2017 Feb 1;26(3):467-478.
Sequence Similarity
Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
Tissue Specificity
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
Post-translational Modification
Glycosylated.
Subcellular Location
Plasma membrane.
Synonyms
Equilibrative NBMPR-sensitive nucleoside transporter antibody
equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter antibody
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter antibody
Equilibrative nucleoside transporter 1 antibody
es-type antibody
MGC1465 antibody
MGC3778 antibody
Nucleoside transporter antibody
Nucleoside transporter, es-type antibody
OTTHUMP00000016506 antibody
ExpandImages
-
Western blot analysis of ENT1 on Human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
-
ICC staining of ENT1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-23, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
Flow cytometric analysis of ENT1 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
