SARS-CoV-2 Nucleocapsid Protein Rabbit Polyclonal Antibody
Catalog# ER1902-39
SARS-CoV-2 Nucleocapsid Protein Rabbit Polyclonal Antibody
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ELISA
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WB
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SARS-CoV-2
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unconjugated
Overview
Product Name
SARS-CoV-2 Nucleocapsid Protein Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Full length of SARS-CoV-2 Nucleocapsid Protein.
Species Reactivity
SARS-CoV-2
Validated Applications
ELISA, WB
Molecular Weight
Predicted band size: 46 kDa
Positive Control
Recombinant SARS-CoV-2 Nucleocapsid Protein.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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ELISA
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1:1,000-1:5,000
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WB
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1:500-1:2,000
Target
Function
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Background References
1. Wu,F. A new coronavirus associated with human respiratory disease in China. Nature 579 (7798), 265-269 (2020).
Subcellular Location
Virion, Host cytoplasm.
Synonyms
SARS-CoV-2 Nucleocapsid Protein antibody
SARS-CoV-2 NP antibody
nucleocapsid protein antibody
novel coronavirus N Protein antibody
novel coronavirus Nucleocapsid Protein antibody
2019-nCoV Nucleoprotein antibody
2019-nCoV N antibody
2019nCoV N antibody
2019-nCoV N Protein antibody
2019 peak N Protein antibody
ExpandImages
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ELISA analysis with anti-SARS-CoV-2 Nucleocapsid Proein antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-SARS-CoV-2 Nucleocapsid Protein antibody was used as the capture antigen for ELISA.
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Western blot using anti-SARS-CoV-2 Nucleocapsid Protein antibody shows detection of a 48 kDa band corresponding to the protein.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"