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ATP8A1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-61

ATP8A1 Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ATP8A1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human ATP8A1 aa 410-637 / 1,164.

Species Reactivity

Human, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 131 kDa

Positive Control

MCF-7 cell lysate, rat brain tissue lysate, MCF-7.

Conjugation

unconjugated

RRID

AB_3069445

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells.

Background References

1. van der Velden L.et.al.Heteromeric interactions required for abundance and subcellular localization of human CDC50 proteins and class 1 P4-ATPases.J. Biol. Chem. 285:40088-40096(2010).

Sequence Similarity

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.

Tissue Specificity

Found in most adult tissues except liver, testis and placenta. Most abundant in heart, brain and skeletal muscle. Also detected in fetal tissues. Isoform 1 is only detected in brain, skeletal muscle and heart and is the most abundant form in skeletal muscle.

Subcellular Location

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane.

UNIPROT

SwissProt: Q9Y2Q0 Human

SwissProt: A0A8I5ZXX6 Rat

Synonyms

Phospholipid-transporting ATPase IA antibody

ATPase class I type 8A member 1 antibody

Chromaffin granule ATPase II antibody

P4-ATPase flippase complex alpha subunit ATP8A1 antibody

ATP8A1 antibody

Images

  • Western blot analysis of ATP8A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER1902-61" style="font-weight: bold;text-decoration: underline;">ER1902-61</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: MCF-7 cell lysate<br />Lane 2: Rat brain tissue lysate

    Western blot analysis of ATP8A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell lysate
    Lane 2: Rat brain tissue lysate

  • Flow cytometric analysis of ATP8A1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER1902-61" style="font-weight: bold;text-decoration: underline;">ER1902-61</a>, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).

    Flow cytometric analysis of ATP8A1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-61, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation