MSR1 / CD204 Rabbit Polyclonal Antibody
Catalog# ER1902-71
MSR1 / CD204 Rabbit Polyclonal Antibody
-
WB
-
FC
-
Human
-
unconjugated
Overview
Product Name
MSR1 / CD204 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human MSR1 aa 51-250.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size 49 kDa
Positive Control
Hela cell lysates, SHSY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
FC
-
1:50-1:100
Target
Function
This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. They were thought to be expressed macrophage-specific, but recently shown to be present on different dendritic cells classes, too. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.
Background References
1. Matsumoto A.et.al.Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions.Proc. Natl. Acad. Sci. U.S.A. 87:9133-9137(1990).
Tissue Specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. Isoform I and isoform II are expressed in the liver, placenta and brain.
Subcellular Location
Membrane.
UNIPROT
Synonyms
CD204 antibody
CD204 antigen antibody
CD24 antibody
Macrophage acetylated LDL receptor I and II antibody
Macrophage scavenger receptor 1 antibody
Macrophage scavenger receptor type III antibody
Macrophage scavenger receptor types I and II antibody
Msr 1 antibody
MSR1 antibody
MSRE_HUMAN antibody
ExpandImages
-
Western blot analysis of MSR1 / CD204 on hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
-
Flow cytometric analysis of MSR1 / CD204 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-71, 1/100) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"