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MARCH9 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1903-04

MARCH9 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

MARCH9 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human MARCH9 aa 21-70.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 38 kDa

Positive Control

MCF7 cell lysate, A549 cell lysate.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Background References

1. Lin M et al. MARCH9 Mediates NOX2 Ubiquitination to Alleviate NLRP3 Inflammasome-Dependent Pancreatic Cell Pyroptosis in Acute Pancreatitis. Pancreas. 2023 Jan

2. Liu H et al. The role of MARCH9 in colorectal cancer progression. Front Oncol. 2022 Sep

Subcellular Location

Golgi apparatus membrane, Lysosome membrane.

UNIPROT

SwissProt: Q86YJ5 Human

Synonyms

BC019560 antibody

E3 ubiquitin protein ligase MARCH9 antibody

FLJ36578 antibody

MARCH IX antibody

Membrane associated RING CH protein IX antibody

membrane associated ring finger (C3HC4) 9 antibody

Membrane associated RING finger protein 9 antibody

RING finger protein 179 antibody

RNF179 antibody

Images

  • Western blot analysis of MARCH9 on different lysates with Rabbit anti-MARCH9 antibody (<a href="/products/ER1903-04" style="font-weight: bold;text-decoration: underline;">ER1903-04</a>) at 1/2,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: A549 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 20 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1903-04" style="font-weight: bold;text-decoration: underline;">ER1903-04</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MARCH9 on different lysates with Rabbit anti-MARCH9 antibody (ER1903-04) at 1/2,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: A549 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 45 kDa

    Exposure time: 20 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1903-04) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"