BRD8 Rabbit Polyclonal Antibody
Catalog# ER1904-32
BRD8 Rabbit Polyclonal Antibody
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IHC-P
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IF-Tissue
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Rat
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unconjugated
Overview
Product Name
BRD8 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human BRD8 331-430/1235
Species Reactivity
Rat
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
135 KDa
Positive Control
Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:100-500
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IF-tissue
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1:100-500: 1:5000-10000
Target
Function
BRD8 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein.
Background References
暂无
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Brd8 antibody
BRD8_HUMAN antibody
Bromodomain containing 8 antibody
Bromodomain-containing protein 8 antibody
p120 antibody
Skeletal muscle abundant protein 2 antibody
Skeletal muscle abundant protein antibody
SMAP antibody
SMAP2 antibody
Thyroid hormone receptor coactivating protein 120kDa antibody
ExpandImages
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Sample:
Hela(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
Primary: Anti- BRD8 (ER1904-32) at 1/1000 dilution
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 130 kD
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"