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C3a R Rabbit Polyclonal Antibody

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Catalog# ER1904-90

C3a R Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Pig

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

C3a R Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human C3a R aa 439-482.

Species Reactivity

Human, Mouse, Pig

Validated Applications

WB

Molecular Weight

Predicted band size: 54 kDa

Positive Control

HL-60 cell lysate, PANC-1 cell lysate, A549 cell lysate, RAW264.7 cell lysate, Mouse lung tissue lysate.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

The C3a receptor also known as complement component 3a receptor 1 (C3AR1) is a G protein-coupled receptor protein involved in the complement system. The receptor binds to complement component C3a, although there is limited evidence that this receptor also binds C4a in lesser mammals. This has yet to be proven true in humans. C3a receptor modulates immunity, arthritis, diet-induced obesity and cancers.

Background References

1. Corcoran JA et al. C3aR plays both sides in regulating resistance to bacterial infections. PLoS Pathog. 2022 Aug

2. Gedam M et al. Complement C3aR depletion reverses HIF-1alpha-induced metabolic impairment and enhances microglial response to Abeta pathology. J Clin Invest. 2023 Jun

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q16581 Human

SwissProt: O09047 Mouse

Synonyms

AZ 3B antibody

AZ3B antibody

C3a anaphylatoxin chemotactic receptor antibody

C3A R antibody

C3A R1 antibody

C3a Receptor antibody

C3a-R antibody

C3AR antibody

C3AR_HUMAN antibody

C3AR1 antibody

Expand

Images

  • Western blot analysis of C3a R on different lysates with Rabbit anti-C3a R antibody (<a href="/products/ER1904-90" style="font-weight: bold;text-decoration: underline;">ER1904-90</a>) at 1/5,000 dilution.<br /><br />Lane 1: HL-60 cell lysate<br />Lane 2: PANC-1 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: Mouse lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1904-90" style="font-weight: bold;text-decoration: underline;">ER1904-90</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of C3a R on different lysates with Rabbit anti-C3a R antibody (ER1904-90) at 1/5,000 dilution.

    Lane 1: HL-60 cell lysate
    Lane 2: PANC-1 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: Mouse lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1904-90) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Application: Western blot<br /><br />Species: Mouse, Pig<br />Sample: Brain<br /><br />Antibody concentration: 1: 5,000<br /><br />Date by conrtesy of: Prof. Sen Yan, Guangdong-HongKong-Macau Institutue of CNS Regeneration (GHMICR), Jinan University

    Application: Western blot

    Species: Mouse, Pig
    Sample: Brain

    Antibody concentration: 1: 5,000

    Date by conrtesy of: Prof. Sen Yan, Guangdong-HongKong-Macau Institutue of CNS Regeneration (GHMICR), Jinan University

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation