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CD80 Rabbit Polyclonal Antibody

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Catalog# ER1905-99

CD80 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD80 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from rat CD80: 251-321/321

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

30 kDa

Positive Control

Expressed on activated B-cells, macrophages and dendritic cells.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Background References

暂无

Subcellular Location

Membrane; Single-pass type I membrane protein.

UNIPROT

SwissProt: P33681 Human

SwissProt: Q00609 Mouse

SwissProt: G3V671 Rat

Synonyms

Activation B7-1 antigen

B lymphocyte activation antigen B7

B7

B7-1

B7-1 antigen

BB1

CD28 antigen ligand 1

CD28LG

CD28LG1

CD80

Expand

Images

  • Sample: <br />Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug<br />Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug<br />Lane 3: Thymus (Rat) Lysate at 40 ug<br />Lane 4: Raji (Human) Cell Lysate at 30 ug<br />Lane 5: K562 (Human) Cell Lysate at 30 ug<br />Primary: Anti-CD80 (<a href="/products/ER1905-99" style="font-weight: bold;text-decoration: underline;">ER1905-99</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 60-65 kD<br />Observed band size: 60 kD

    Sample:
    Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug
    Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug
    Lane 3: Thymus (Rat) Lysate at 40 ug
    Lane 4: Raji (Human) Cell Lysate at 30 ug
    Lane 5: K562 (Human) Cell Lysate at 30 ug
    Primary: Anti-CD80 (ER1905-99) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60-65 kD
    Observed band size: 60 kD

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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