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CD86 Rabbit Polyclonal Antibody

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Catalog# ER1906-01

CD86 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD86 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from the middle of rat CD86:140-175/313 <Extracellular>

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, FC

Molecular Weight

38 KDa

Positive Control

Expressed by activated B-lymphocytes and monocytes.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

  • FC

  • 2μg/Test

Target

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

Background References

暂无

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

UNIPROT

SwissProt: P42081 Human

SwissProt: P42082 Mouse

SwissProt: O35531 Rat

Synonyms

Activation B7-2 antigen 3 antibody

Activation B7-2 antigen antibody

B-lymphocyte activation antigen B7-2 2 antibody

B-lymphocyte activation antigen B7-2 antibody

B7 2 antibody

B7 antibody

B7-2 antibody

B7.2 antibody

B70 antibody

B72 antibody

Expand

Images

  • Sample: <br />Lane 1: HepG2 (Human) Cell Lysate at 30 ug<br />Lane 2: U937 (Human) Cell Lysate at 30 ug<br />Lane 3: Spleen (Rat) Lysate at 40 ug<br />Lane 4: Spleen (Mouse) Lysate at 40 ug<br />Lane 5: HL-60 (Human) Cell Lysate at 30 ug<br />Primary: Anti-CD86 (<a href="/products/ER1906-01" style="font-weight: bold;text-decoration: underline;">ER1906-01</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 72-74 kD<br />Observed band size: 72 kD

    Sample:
    Lane 1: HepG2 (Human) Cell Lysate at 30 ug
    Lane 2: U937 (Human) Cell Lysate at 30 ug
    Lane 3: Spleen (Rat) Lysate at 40 ug
    Lane 4: Spleen (Mouse) Lysate at 40 ug
    Lane 5: HL-60 (Human) Cell Lysate at 30 ug
    Primary: Anti-CD86 (ER1906-01) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 72-74 kD
    Observed band size: 72 kD

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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