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Egr1 Rabbit Polyclonal Antibody

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Catalog# ER1907-98

Egr1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Egr1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from human Egr-1 401-500/453

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell, IF-Tissue, FC

Molecular Weight

60 KDa

Positive Control

Mouse small intestine, U-937 cells, Mouse Lung Lysate, rat liver tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-cell

  • 1:100

  • IF-tissue

  • 1:100-500

  • FC

  • 2ug/test

Target

Function

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary. Regulates the amplitude of the expression rhythms of clock genes: ARNTL/BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene ARNTL/BMAL1 in the suprachiasmatic nucleus (SCN).

Background References

暂无

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P18146 Human

Synonyms

AT225 antibody

Early growth response 1 antibody

Early growth response protein 1 antibody

EGR 1 antibody

EGR-1 antibody

EGR1 antibody

EGR1_HUMAN antibody

G0S30 antibody

KROX 24 antibody

KROX24 antibody

Expand

Images

  • Sample: <br />Lung (Mouse) Lysate at 40 ug<br />Primary: Anti-Egr1 (<a href="/products/ER1907-98" style="font-weight: bold;text-decoration: underline;">ER1907-98</a>) at 1/300 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 60 kD<br />Observed band size: 60 kD

    Sample:
    Lung (Mouse) Lysate at 40 ug
    Primary: Anti-Egr1 (ER1907-98) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 60 kD

  • Sample: <br />Liver (Rat) Lysate at 40 ug<br />Primary: Anti- Egr1 (<a href="/products/ER1907-98" style="font-weight: bold;text-decoration: underline;">ER1907-98</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 60 kD<br />Observed band size: 60 kD

    Sample:
    Liver (Rat) Lysate at 40 ug
    Primary: Anti- Egr1 (ER1907-98) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 60 kD

  • Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; <br />Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; <br />Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(<a href="/products/ER1907-98" style="font-weight: bold;text-decoration: underline;">ER1907-98</a>) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(ER1907-98) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

  • Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Egr1) Polyclonal Antibody, Unconjugated (<a href="/products/ER1907-98" style="font-weight: bold;text-decoration: underline;">ER1907-98</a>) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Egr1) Polyclonal Antibody, Unconjugated (ER1907-98) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

  • A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Egr1) polyclonal Antibody, Unconjugated (<a href="/products/ER1907-98" style="font-weight: bold;text-decoration: underline;">ER1907-98</a>) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

    A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Egr1) polyclonal Antibody, Unconjugated (ER1907-98) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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EGR1 Recombinant Rabbit Monoclonal Antibody [JE57-62]

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