GSDMC Rabbit Polyclonal Antibody
Catalog# ER1910-25
GSDMC Rabbit Polyclonal Antibody
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WB
-
IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
GSDMC Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human GSDMC 1-100/508
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
58 KDa
Positive Control
Expressed primarily in trachea and spleen.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:500-2000
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IHC-P
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1:100-500
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IF-tissue
-
1:100-500
Target
Function
The Gasdermin (GSDM) family consists of Gasdermin A (GSDMA), Gasdermin B (GSDMB), Gasdermin C (GSDMC), Gasdermin D (GSDMD), Gasdermin E (GSDME) and Pejvakin (PJVK). GSDMD is activated by inflammasome-associated inflammatory caspases. Cleavage of GSDMD by human or mouse caspase-1, human caspase-4, human caspase-5, and mouse caspase-11 liberates the N-terminal effector domain from the C-terminal inhibitory domain. The N-terminal domain oligomerizes in the cell membrane and forms a pore of 10-16 nm in diameter, through which substrates of a smaller diameter, such as interleukin-1β and interleukin-18, are secreted. The increasing abundance of membrane pores ultimately leads to membrane rupture and pyroptosis, releasing the entire cellular content. Other than GSDMD, the N-terminal domain of all GSDMs, with the exception of PJVK, have the ability to form pores. There is evidence to suggest that GSDMB and GSDME are cleaved by apoptotic caspases. Here, we review the mechanistic functions of GSDM proteins with respect to their expression and signaling profile in the cell, with more focused discussions on inflammasome activation and cell death.
Background References
暂无
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Gasdermin C antibody
Gasdermin-C antibody
GSDMC antibody
GSDMC_HUMAN antibody
GSDMC1 antibody
Melanoma derived leucine zipper
extra nuclear factor antibody
Melanoma-derived leucine zipper-containing extranuclear factor antibody
MLZE antibody
Images
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Sample:
EC-9706 Cell (Human) Lysate at 40 ug
FHC Cell (Human) Lysate at 40 ug
Primary: Anti- GSDMC (ER1910-25) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD -
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human A549 cell lysates
Lane 3: Human 293T cell lysates
Primary: Anti-GSDMC (ER1910-25) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 47 kDa -
Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSDMC) Polyclonal Antibody, Unconjugated (ER1910-25) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
GSDMC Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
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