IGFBP3 Rabbit Polyclonal Antibody
Catalog# ER1911-12
IGFBP3 Rabbit Polyclonal Antibody
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IHC-P
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IF-Tissue
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Human
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Rat
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unconjugated
Overview
Product Name
IGFBP3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human IGFBP3 186-292/292
Species Reactivity
Human, Rat
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
30-35 KDa
Positive Control
Expressed by most tissues. Present in plasma.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
Target
Background References
暂无
Subcellular Location
Secreted.
UNIPROT
Synonyms
Acid stable subunit of the 140 K IGF complex antibody
Binding protein 29 antibody
Binding protein 53 antibody
BP 53 antibody
BP53 antibody
Growth hormone dependent binding protein antibody
IBP 3 antibody
IBP-3 antibody
IBP3 antibody
IBP3_HUMAN antibody
ExpandImages
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated(ER1911-12) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IGFBP3 Polyclonal Antibody, Unconjugated(ER1911-12) 1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37℃. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human IGFBP3 Recombinant Rabbit Monoclonal Antibody [PSH12-17] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
Human IGFBP3 Recombinant Rabbit Monoclonal Antibody [PSH12-16] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
