PDE4D Rabbit Polyclonal Antibody
Catalog# ER1914-89
PDE4D Rabbit Polyclonal Antibody
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WB
-
FC
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Mouse
-
Rat
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unconjugated
Overview
Product Name
PDE4D Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human PDE4D 401-550/809
Species Reactivity
Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
90 KDa
Positive Control
Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:500-2000
-
FC
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1μg/Test
Target
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Background References
暂无
Subcellular Location
Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
UNIPROT
Synonyms
DPDE3 antibody
hPDE4D3 antibody
HSPDE4D antibody
PDE43 antibody
PDE4D antibody
PDE4DN2 antibody
Phosphodiesterase 4D antibody
Phosphodiesterase 4D cAMP-specific antibody
STRK1 antibody
Images
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Sample:Brain(Mouse) Lysate at 30 ug
Primary: Anti-PDE4D (ER1914-89) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90kD -
Sample:Brain(Mouse) Lysate at 30 ug
Primary: Anti-PDE4D (ER1914-89) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 100 kD -
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- PDE4D (ER1914-89) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90kD
Observed band size: 90 kD -
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
PDE4D Mouse Monoclonal Antibody [A7F3]
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
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