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RIP2 Rabbit Polyclonal Antibody

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Catalog# ER1915-87

RIP2 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

RIP2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from human RIP2 401-540/540

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue, FC

Molecular Weight

60 KDa

Positive Control

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage Buffer

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

  • FC

  • 2ug/Test

Target

Function

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Background References

暂无

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O43353 Human

Synonyms

CARD 3 antibody

CARD carrying kinase antibody

CARD containing ICE associated kinase antibody

CARD containing IL 1 beta ICE kinase antibody

CARD containing IL1 beta ICE kinase antibody

CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody

CARD containing interleukin 1 beta converting enzyme associated kinase antibody

CARD-containing IL-1 beta ICE-kinase antibody

CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody

CARD3 antibody

Expand

Images

  • Sample: <br />Hela(Human) Cell Lysate at 30 ug<br />Primary: Anti-RIP2 (<a href="/products/ER1915-87" style="font-weight: bold;text-decoration: underline;">ER1915-87</a>) at 1/1000 dilution<br />Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution<br />Predicted band size: 60 kD<br />Observed band size: 60 kD

    Sample:
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti-RIP2 (ER1915-87) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 60 kD
    Observed band size: 60 kD

  • Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated (<a href="/products/ER1915-87" style="font-weight: bold;text-decoration: underline;">ER1915-87</a>) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated (ER1915-87) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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RIPK2 Recombinant Rabbit Monoclonal Antibody [JE51-90]

Application: WB

Reactivity: Human

Conjugate: unconjugated