SIRP Alpha Rabbit Polyclonal Antibody
Catalog# ER1916-61
SIRP Alpha Rabbit Polyclonal Antibody
-
WB
-
FC
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
SIRP Alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human CD172a 151-250/504
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
56 KDa
Positive Control
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-2000
-
FC
-
1μg /test
Target
Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Background References
暂无
Subcellular Location
Membrane; Single-pass type I membraneprotein.
UNIPROT
Synonyms
Signal regulatory protein alpha type 1
BIT
Brain Ig like molecule with tyrosine based activation motifs
Brain immunoglobulin like molecule with tyrosine based activation motifs
CD172 antigen like family member A
CD172a
CD172a antigen
Inhibitory receptor SHPS1
Macrophage fusion receptor
MFR
ExpandImages
-
Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Mouse Spleen Lysates
Lane 4: Mouse Placenta Lysates
Lane 5: Rat Brain Lysates
Lane 6: Rat Cerebellum Lysates
Primary: Anti-SIRP Alpha(ER1916-61) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kDa
Observed band size: 56kDa -
Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- SIRP antibody(ER1916-61), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
SIRP alpha Mouse Monoclonal Antibody [A3E3]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated