TNF alpha Rabbit Polyclonal Antibody
Catalog# ER1919-22
TNF alpha Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
TNF alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human TNF-alpha:181-233/233 <Extracellular>
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
17/26 kDa
Positive Control
Liver (mouse) Lysate, Diseased lung(mouse).
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-2000: 1:5000-10000
Target
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.
Background References
1. Friedmann E. et al. SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production. Nat. Cell Biol. 8:843-848(2006)
2. Nie H. et al. Phosphorylation of FOXP3 controls regulatory T cell function and is inhibited by TNF-alpha in rheumatoid arthritis. Nat. Med. 19:322-328(2013)
Subcellular Location
Cell membrane, Secreted.
Synonyms
APC1 antibody
APC1 protein antibody
Cachectin antibody
DIF antibody
Differentiation inducing factor antibody
Macrophage cytotoxic factor antibody
Tnf antibody
TNF superfamily member 2 antibody
TNF superfamily
member 2 antibody
ExpandImages
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Sample: Diseased lung(mouse) at 30ug;
Primary: Anti-TNF alpha (ER1919-22) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size :17/28 kD
Observed band size :17/28 kD -
Sample: Liver (mouse) Lysate at 40 ug
Primary: Anti-TNF alpha(ER1919-22)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/26kD
Observed band size: 25 kD -
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Primary:
Anti-TNF alpha (ER1919-22) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36/17/26 kD
Observed band size: 36 kD
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Mechanism of Qufeng Huoxue decoction in treating paclitaxel-induced peripheral neuropathy based on network pharmacology and molecular docking
Journal: Neuropeptides
DOI: 10.1016/j.npep.2026.102608
IF: 2.7
Application: WB
Reactivity: Mouse
Publish date: 2026 Mar
-
Necroptosis mediated by FKBP5 in alveolar fibroblasts drives Pseudomonas aeruginosa-induced acute lung injury
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2026.116368
IF: 4.7
Application: WB
Reactivity: Mouse,Human
Publish date: 2026 Feb
-
CircCdh7 induces astrogliosis and neuroinflammation to trigger hypertensive effects in the rostral ventrolateral medulla
Journal: Cell & Bioscience
DOI: 10.1186/s13578-025-01480-0
IF: 6.2
Application: WB
Reactivity: Rat
Publish date: 2025 Oct
-
Network pharmacology, molecular docking, molecular dynamics simulation, and in vivo experiments elucidate the potential mechanisms of berberine against liver injury
Journal: Naunyn-Schmiedebergs Archives Of Pharmacology
DOI: 10.1007/s00210-025-04697-5
IF: 3.1
Application: WB
Reactivity: Mouse
Publish date: 2025 Oct
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Double Cross-Linked Hydrogel for Intra-articular Injection as Modality for Macrophages Metabolic Reprogramming and Therapy of Rheumatoid Arthritis
Journal: Advanced Functional Materials
DOI: 10.1002/adfm.202502880
IF: 18.5
Application: WB
Reactivity: Mouse
Publish date: 2025 Mar
-
FKBP5 Mediates Alveolar Fibroblast Necroptosis During Acute Respiratory Distress Syndrome
Journal: Cell Proliferation
DOI: 10.1111/cpr.70075
IF: 5.6
Application: WB
Reactivity: Mouse,Human
Publish date: 2025 Jun
-
Heparan sulfate acts in synergy with tight junction through STAT3 signaling to maintain the endothelial barrier and prevent lung injury development
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
DOI: 10.1016/j.intimp.2024.113957
IF: 4.7
Application: WB
Reactivity: Mouse
Publish date: 2025 Jan
-
Ponicidin ameliorates Alzheimer's disease through dual inhibition of RIPK1-mediated neuroinflammation and necroptosis
Journal: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.116095
IF: 4.7
Application: WB
Reactivity: Mouse
Publish date: 2025 Dec
-
Human Amniotic Epithelial Stem Cells Alleviate Autoimmune Premature Ovarian Insufficiency in Mice by Targeting Granulosa Cells via AKT/ERK Pathways
Journal: Stem Cell Reviews And Reports
DOI:
IF: 4.8
Application: IHC-P
Reactivity: Mouse
Publish date: 2024 Jun
-
Baicalin-Geniposide glycosides inhibit PM2.5-induced brain damage in rats via the 5-LOX/LTB4 pathway
Journal: Molecular Immunology
DOI: 10.1016/j.molimm.2024.12.005
IF: 3.2
Application: WB
Reactivity: Mouse
Publish date: 2024 Dec
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A Rapid Self-Assembling Peptide Hydrogel for Delivery of TFF3 to Promote Gastric Mucosal Injury Repair
Journal: Molecules
DOI:
IF: 4.2
Application: WB
Reactivity: Human
Publish date: 2024 Apr
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Identification of circRNA-miRNA-mRNA network as biomarkers for interstitial cystitis/bladder pain syndrome.
Journal: Aging-Us
DOI: 10.18632/aging.205170
IF: 5.2
Application: WB
Reactivity: Human
Publish date: 2023 Nov
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Potential contribution of early endothelial progenitor cell (eEPC)-to-macrophage switching in the development of pulmonary plexogenic lesion
Journal: Respiratory Research
DOI:
IF: 7.162
Application: IHC-P
Reactivity: Chicken
Publish date: 2022 Oct
-
Rosuvastatin Nanomicelles Target Neuroinflammation and Improve Neurological Deficit in a Mouse Model of Intracerebral Hemorrhage. International journal of nanomedicine, 16, 2933–2947.
Journal: International Journal Of Nanomedicine
DOI:
IF: 6.4
Application: WB,IHC-P
Reactivity: Mouse
Publish date: 2021 Apr
-
Anti-foodborne enteritis effect of galantamine potentially via acetylcholine anti-inflammatory pathway in fish
Journal: Fish & Shellfish Immunology
DOI:
IF: 3.30
Application: WB
Reactivity: Zebrafish
Publish date: 2020 Feb
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