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FYCO1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-04

FYCO1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

FYCO1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human FYCO1 aa 1-200.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 167 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate.

Conjugation

unconjugated

RRID

AB_3073452

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

This gene encodes a protein that contains a RUN domain, FYVE-type zinc finger domain and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P) and the autophagosome marker LC3. Mutations in this gene are a cause of autosomal recessive congenital cataract-2 (CATC2).

Background References

1. Saridaki T. et. al. FYCO1 mediates clearance of α-synuclein aggregates through a Rab7-dependent mechanism. J Neurochem. 2018 Aug

2. Dionne LK. et. al. FYCO1 regulates accumulation of post-mitotic midbodies by mediating LC3-dependent midbody degradation. J Cell Sci. 2017 Dec

Subcellular Location

Lysosome, Endosome, autophagosome.

UNIPROT

SwissProt: Q9BQS8 Human

SwissProt: Q8VDC1 Mouse

Entrez Gene: 301085 Rat

Synonyms

CATC2 antibody

CTRCT18 antibody

DKFZp779K1152 antibody

FLJ13335 antibody

FYCO1 antibody

FYCO1_HUMAN antibody

FYVE and coiled coil domain containing 1 antibody

FYVE and coiled coil domain containing protein 1 antibody

FYVE and coiled-coil domain-containing protein 1 antibody

MGC126517 antibody

Expand

Images

  • Western blot analysis of FYCO1 on HeLa (Human cervical adenocarcinoma cell) cell lysate with Rabbit anti-FYCO1 antibody (<a href="/products/ER2001-04" style="font-weight: bold;text-decoration: underline;">ER2001-04</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER2001-04" style="font-weight: bold;text-decoration: underline;">ER2001-04</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 167 kDa<br />Observed band size: 167 kDa

    Western blot analysis of FYCO1 on HeLa (Human cervical adenocarcinoma cell) cell lysate with Rabbit anti-FYCO1 antibody (ER2001-04) at 1/5,000 dilution.

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER2001-04, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 167 kDa
    Observed band size: 167 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"