Myomegalin Rabbit Polyclonal Antibody
Catalog# ER2001-16
Myomegalin Rabbit Polyclonal Antibody
-
WB
-
Mouse
-
unconjugated
Overview
Product Name
Myomegalin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within rat Myomegalin aa 2130-2190.
Species Reactivity
Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 251 kDa
Positive Control
NIH/3T3 cell lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
Functions as an anchor sequestering components of the cAMP-dependent pathway to Golgi and/or centrosomes . May participate in microtubule dynamics, promoting microtubule assembly, in an isoform-specific manner. Depending upon the cell context, may act at the level of the Golgi apparatus or that of the centrosome. In complex with AKAP9, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. In complex with AKAP9, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension from the centrosome to the cell periphery, a crucial process for directed cell migration, mitotic spindle orientation and cell-cycle progression (By similarity).
Background References
1. Verde I. et. al. Myomegalin is a novel protein of the Golgi/centrosome that interacts with a cyclic nucleotide phosphodiesterase. J. Biol. Chem. 276:11189-11198(2001).
Subcellular Location
Cytoplasm, Cytoskeleton, Golgi apparatus.
Synonyms
Cardiomyopathy associated protein 2 antibody
Cardiomyopathy-associated protein 2 antibody
CMYA2 antibody
MMGL antibody
MYOME_HUMAN antibody
Myomegalin antibody
Pde4dip antibody
Phosphodiesterase 4D interacting protein antibody
Phosphodiesterase 4D-interacting protein antibody
Images
-
Western blot analysis of Myomegalin on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"