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ARF16 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-21

ARF16 Rabbit Polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Rice

Conjugation

  • unconjugated

Overview

Product Name

ARF16 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Japonica rice aa 580-640.

Species Reactivity

Rice

Validated Applications

WB, ELISA

Molecular Weight

Predicted band size: 117 kDa

Positive Control

Rice tissue lysates.

Conjugation

unconjugated

RRID

AB_3069500

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • ELISA

  • 1:5,000

Target

Function

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).

Background References

1. Wang D. et. al. Genome-wide analysis of the auxin response factors (ARF) gene family in rice (Oryza sativa). Gene 394:13-24(2007).

Subcellular Location

Nucleus.

UNIPROT

SwissProt: A3B9A0 Rice

Synonyms

Auxin respoARF16nse factor 16 antibidy

ARF16 antibody

Images

  • Western blot analysis of ARF16 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-21" style="font-weight: bold;text-decoration: underline;">ER2001-21</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ARF16 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"