AKR1B1 Rabbit Polyclonal Antibody
Catalog# ER2001-28
AKR1B1 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
-
Zebrafish
Predicted species support after-sales service
-
unconjugated
Overview
Product Name
AKR1B1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human AKR1B1 aa 200-260.
Species Reactivity
Human (Predicted: Mouse, Rat, Zebrafish)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
36 kDa
Positive Control
A549 cell lysate, Siha cell lysate, Siha, human seminal vesicle tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:100-1:500
Target
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia . Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal . Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadi
Background References
1. Liu S.Q. et. al. Identification of the reactive cysteine residue in human placenta aldose reductase.Biochim. Biophys. Acta 1164:268-272(1993).
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
ADR antibody
AKR1B 1 antibody
Akr1b1 antibody
Aldehyde reductase 1 antibody
Aldehyde reductase antibody
Aldo keto reductase family 1, member B1 antibody
Aldo-keto reductase family 1 member B1 antibody
aldo-keto reductase family 1, member B1 (aldose reductase) antibody
Aldose reductase antibody
aldr 1 antibody
ExpandImages
-
Western blot analysis of AKR1B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Siha cell lysate -
ICC staining of AKR1B1 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using anti-AKR1B1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-28, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"