P2RX5 Rabbit Polyclonal Antibody
Catalog# ER2001-36
P2RX5 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Rat
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Mouse
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unconjugated
Overview
Product Name
P2RX5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within rat P2RX5 aa 400-455.
Species Reactivity
Rat, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
51 kDa
Positive Control
Rat brain tissue lysate, rat heart tissue lysate, mouse brain tissue, rat brain tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:500-1:1,000
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IHC-P
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1:50-1:200
Target
Function
P2X purinoceptor 5 is a protein that in humans is encoded by the P2RX5 gene. The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Four transcript variants encoding distinct isoforms have been identified for this gene.
Background References
1. Greig AV. et. al. Purinergic receptors are part of a functional signaling system for proliferation and differentiation of human epidermal keratinocytes. J. Invest. Dermatol. 120 : 1007–15(2003).
Subcellular Location
Membrane.
Synonyms
ATP receptor antibody
ATP receptor subunit antibody
Ionotropic ATP receptor P2X5 antibody
LRH 1 antibody
LRH1 antibody
Lymphoid restricted histocompatibility antigen 1 antibody
MGC47755 antibody
P2rx5 antibody
P2RX5_HUMAN antibody
P2X purinoceptor 5 antibody
ExpandImages
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Western blot analysis of P2RX5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-36, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"