TMEM119 Rabbit Polyclonal Antibody
Catalog# ER2001-37
TMEM119 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TMEM119 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Transmembrane protein 119 aa 120-170.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 29 kDa
Positive Control
SH-SY5Y (Human neuroblastoma cell) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C6 (Rat glioma cell) cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
Target
Function
Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts . May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation (By similarity).
Background References
1. Kanamoto T. et. al. Isolation and characterization of a novel plasma membrane protein, osteoblast induction factor (obif), associated with osteoblast differentiation. BMC Dev. Biol. 9:70-70(2009).
Subcellular Location
Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane.
UNIPROT
Synonyms
OBIF antibody
Osteoblast induction factor antibody
PSEC0199 antibody
Transmembrane protein 119 antibody
UNQ731/PRO1415 antibody
Images
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Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (ER2001-37) at 1/2,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER2001-37, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 29 kDa
Observed band size: 35 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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