Smoothelin Rabbit Polyclonal Antibody
Catalog# ER2001-40
Smoothelin Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Smoothelin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Smoothelin aa 300-487.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
99 kDa
Positive Control
PC-3M cell lysates, human uterine fibroids tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50
Target
Function
Structural protein of the cytoskeleton. This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Background References
1. van der Loop F T L et al. Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells. J Cell Biol 134:401-411 (1996).
2. Rensen S S et al. Expression of the smoothelin gene is mediated by alternative promoters. Cardiovasc Res 55:850-863 (2002).
Subcellular Location
Cytoplasm, Cytoskeleton.
UNIPROT
Synonyms
FLJ35168 antibody
FLJ35365 antibody
FLJ35620 antibody
FLJ38597 antibody
Smoothelin antibody
SMSMO antibody
Smtn antibody
SMTN_HUMAN antibody
Images
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Western blot analysis of Smoothelin on PC-3M cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human uterine fibroids tissue using anti-Smoothelin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER2001-40) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"