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GRK1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-72

GRK1 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

GRK1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human GRK1 aa 500-563.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 63 kDa

Positive Control

Mouse marrow tissue lysate, HUVEC cell lysate, 293 cell lysate, Rat marrow tissue lysate.

Conjugation

unconjugated

RRID

AB_3069549

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

Target

Function

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).

Background References

1. Horner T.J. et. al. Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities. J. Biol. Chem. 280:28241-28250(2005).

Subcellular Location

Cell projection, Membrane.

UNIPROT

SwissProt: Q15835 Human

SwissProt: Q9WVL4 Mouse

SwissProt: Q63651 Rat

Synonyms

EC=2.7.11.14 antibody

G protein-coupled receptor kinase 1 antibody

GPRK1 antibody

Grk1 antibody

Rhodopsin kinase antibody

RHOK antibody

RK antibody

RK_HUMAN antibody

Images

  • Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-72" style="font-weight: bold;text-decoration: underline;">ER2001-72</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Mouse marrow tissue lysate<br />Lane 2: HUVEC cell lysate<br />Lane 3: 293 cell lysate<br />Lane 4: Rat marrow tissue lysate

    Western blot analysis of GRK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse marrow tissue lysate
    Lane 2: HUVEC cell lysate
    Lane 3: 293 cell lysate
    Lane 4: Rat marrow tissue lysate

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"