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MuERVL-Gag Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER50102

MuERVL-Gag Rabbit Polyclonal Antibody

Application

  • IF-Cell

  • WB

Reactivity

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MuERVL-Gag Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse MuERVL-Gag aa 541-581/581.

Species Reactivity

Mouse

Validated Applications

IF-Cell, WB

Molecular Weight

Predicted band size: 67 kDa

Positive Control

Mouse embryo, ES-D3 cell lysate, ES-W4 cell lysate.

Conjugation

unconjugated

RRID

AB_2636876

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Cell

  • 1:200

  • WB

  • 1:1,000-1:5,000

Target

Function

A large number of retrotransposons are expressed when the zygotic genome is first transcribed, including the endogenous retroviruses (ERVs), LINE-1 elements, and the non-autonomous SINE elements. At the 2C stage, MuERV-L/MERVL retrovirus-like elements are transiently de-repressed and produce 3% of the transcribed mRNAs. Following the 2C stage, MERVL-retroelement expression is silenced. The newly study discovered that this regulated pattern of MERVL expression overlapped with greater than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to initiate their transcription.

Background References

1. "Cloning of a new murine endogenous retrovirus, MuERV-L, with strong similarity to the human HERV-L element and with a gag coding sequence closely related to the Fv1 restriction gene." Benit L., de Parseval N., Casella J.F., Callebaut I., Cordonnier A., Heidmann T. J. Virol. 71:5652-5657(1997)

2. "Embryonic stem cell potency fluctuates with endogenous retrovirus activity ". Todd S. Macfarlan, et al. Nature. 2012 Jul 5; 487(7405):57-63.

UNIPROT

SwissProt: V9H130 Mouse

Images

  • ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4℃ with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4℃ with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

  • Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (<a href="/products/ER50102" style="font-weight: bold;text-decoration: underline;">ER50102</a>) at 1/1,000 dilution.<br /><br />Lane 1: ES-D3 cell lysate<br />Lane 2: ES-W4 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 67 kDa<br />Observed band size: 67 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER50102" style="font-weight: bold;text-decoration: underline;">ER50102</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MuERVL-Gag on different lysates with Rabbit anti-MuERVL-Gag antibody (ER50102) at 1/1,000 dilution.

    Lane 1: ES-D3 cell lysate
    Lane 2: ES-W4 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

    Exposure time: 46 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER50102) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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